中文
Other Lab Equipments:
Drug Tests / Pharmaceutics
Environmental Monitors
Food & Beverage
Radioactivity / Luminescence
Miscellaneous
- MicroCal PEAQ-DSC Automated
- Product Detail
- Company Profile
- MicroCal PEAQ-DSC Automated
- Gold standard stability-indicating technique requiring minimal assay development
- Direct and label-free measurement of biomolecular native-state stability in solution
- Standard 96-well plate format for high capacity and easy loading, with thermostatically-controlled storage of up to 6 plates
- Fully integrated autosampler enables unattended analysis of up to 50 samples per day
- Automated cell filling and cell cleaning functions permit unattended operation
- Measurement of very tight binding constants, up to 1020M-1
- Powerful MicroCal PEAQ-DSC software minimizes typical data analysis time and includes:
- PEAQ-Compliance: The system is available with Malvern Access Controller (MAC) to limit access to user-defined SOPs and data analysis features. Data can be displayed using the Report Generator with user details and the ability to sign using electronic signatures to facilitate the integration of the workflow into your company’s quality system and aid compliance with 21 CFR Part 11 and Annex 11 regulations. This is provided as an optional accessory
- PEAQ-Performance: Automatically detects and validates system readiness for optimal performance
- PEAQ-Smart (including PEAQ-Finder): For SOP-based operation and data analysis. Provides new algorithms to detect even subtle peaks and shoulders, facilitating the automatic, non-subjective identification of multiple transitions, such as those seen in multi-domain proteins
- PEAQ-Compare: For the quantitative comparison of DSC traces for comparability studies, ideal for batch-to-batch and biosimilarity studies
- Network-ready: email updates sent during the analysis to keep you informed of assay progress
- Telephone and email support.
- Operators who take responsibility for your call and ensure your query is answered.
Gold standard protein stability analysis for the regulated environment
The MicroCal PEAQ-DSC Automated system delivers high throughput, highly sensitive protein stability analysis with low sample consumption, in an automated, integrated platform for increased productivity. All cell filling and cleaning functions are fully automated for walk-away operation.
Data generated by the MicroCal PEAQ-DSC Automated provide critical guidance for biopharmaceutical development in protein engineering, (pre)formulation development, process development, manufacturing change control and biosimilarity and biocomparability studies. Unattended operation enables 24-hour screening, while the integrated software streamlines workflows, facilitates non-subjective data analysis, performance qualification and compliance with 21 CFR Part 11 and Annex 11 regulations, all delivering high integrity data and driving productivity in biopharmaceutical research.
Features and benefits
Differential Scanning Calorimetry (DSC) is a powerful analytical tool for characterizing the thermal stability of proteins and other biomolecules. The technique measures the enthalpy (ΔH) and temperature (Tm) of thermally-induced structural transitions of molecules in solution. This information provides valuable insights into factors that stabilize or destabilize proteins, nucleic acids, micellar complexes and other macromolecular systems. The data are used to predict shelf-life of biomolecular products including biopharmaceuticals to enable batch-to-batch and biosimilar versus innovator molecule comparisons, to develop purification strategies, to characterize and evaluate protein constructs, and to rank the affinities of ligands to their protein targets in small molecule drug discovery programs.
The high-throughput, high-sensitivity MicroCal PEAQ-DSC Automated system features software that streamlines workflows through simplified experimental setup and flexible instrument scheduling. Automated data analysis supports the generation of high integrity thermal stability data and delivers compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems.
Key Features
Differential Scanning Calorimetry (DSC) is a powerful analytical tool for characterizing the thermal stability of proteins and other biomolecules. The technique measures the enthalpy (ΔH) and temperature (Tm) of thermally-induced structural transitions of molecules in solution. This information provides valuable insights into factors that stabilize or destabilize proteins, nucleic acids, micellar complexes and other macromolecular systems. The data are used to predict shelf-life of biomolecular products including biopharmaceuticals to enable batch-to-batch and biosimilar versus innovator molecule comparisons, to develop purification strategies, to characterize and evaluate protein constructs, and to rank the affinities of ligands to their protein targets in small molecule drug discovery programs.
The high-throughput, high-sensitivity MicroCal PEAQ-DSC Automated system features software that streamlines workflows through simplified experimental setup and flexible instrument scheduling. Automated data analysis supports the generation of high integrity thermal stability data and delivers compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems.
Key Features
How it works
Functional structures formed by proteins and other macromolecules often undergo temperature-induced conformational changes, such as unfolding. These changes result in the absorption of heat as a result of the redistribution of non-covalent bonds within the molecule. Differential Scanning Calorimeters measure this heat uptake very precisely.
The thermal core of the MicroCal PEAQ-DSC Automated system contains a sample cell (containing the sample of interest) and a reference cell (containing a matched buffer solution), both of which are housed within an insulating jacket. These two cells are always maintained at the same temperature, and during a measurement, they are heated at a constant scan rate.
As the molecule within the sample cell unfolds, heat is absorbed, creating a temperature difference (ΔT) between the sample cell and the reference cell. This results in a thermal gradient across the Peltier units, creating a proportional voltage, which is in turn converted to power to form a feedback loop to the Peltier units, in order to return ΔT to zero.
Since protein unfolding is an endothermic event, it is observed as a positive displacement in the thermogram. The midpoint of this protein ‘melting’ transition is the Tm, and the area under the curve is the enthalpy (ΔH) of the unfolding process (please see figure below).
Data analysis and measured/derived parameters are described in greater detail on the Differential Scanning Calorimetry technology page.
Functional structures formed by proteins and other macromolecules often undergo temperature-induced conformational changes, such as unfolding. These changes result in the absorption of heat as a result of the redistribution of non-covalent bonds within the molecule. Differential Scanning Calorimeters measure this heat uptake very precisely.
The thermal core of the MicroCal PEAQ-DSC Automated system contains a sample cell (containing the sample of interest) and a reference cell (containing a matched buffer solution), both of which are housed within an insulating jacket. These two cells are always maintained at the same temperature, and during a measurement, they are heated at a constant scan rate.
As the molecule within the sample cell unfolds, heat is absorbed, creating a temperature difference (ΔT) between the sample cell and the reference cell. This results in a thermal gradient across the Peltier units, creating a proportional voltage, which is in turn converted to power to form a feedback loop to the Peltier units, in order to return ΔT to zero.
Since protein unfolding is an endothermic event, it is observed as a positive displacement in the thermogram. The midpoint of this protein ‘melting’ transition is the Tm, and the area under the curve is the enthalpy (ΔH) of the unfolding process (please see figure below).
Data analysis and measured/derived parameters are described in greater detail on the Differential Scanning Calorimetry technology page.
Global networkOur global sales and support network ensures that wherever you are, we’ve got you covered
Free contentFree access to exclusive content: webinars, videos, whitepapers, and much more
Wide range of solutionsWhatever your application or industry, we have the solutions, support, and expertise you need
Quality you can trustInnovative, yet robust and reliable instruments you can trust for continuous, precise, and rapid analysis
Specification
General
Cell
Sample
System
Software
Operating environment
Weight and dimensions
Notes
SupportGeneral
| Technology | Differential Scanning Calorimetry |
|---|---|
| Measurement type |
Temperature midpoint (TM)
Enthalpy (ΔH)
Heat capacity change (ΔCp)
|
| Cell | Capillary |
|---|---|
| Cell material | Tantalum |
| Cell volume | 130 μL |
| Sample capacity | 288 (6 × 96-well plates) |
|---|---|
| Sample volume | 325 µL |
| Typical sample concentration | 0.01 mg/mL – 10 mg/mL 1 |
| Sample throughput | ≤50 samples/day |
| Sample storage temperature range | 4°C - 40°C |
| Noise | 0.05 μCal/°C 2 |
|---|---|
| Baseline repeatability | 1 μCal/°C 2 |
| Response time | 5s 2 |
| Repeatability | <0.2 µCal/°C 3 |
| Reproducibility | <0.08°C St. Dev. TM and <2% RSD on ΔH 4 |
| System reproducibility | <0.1°C St. Dev. TM and <5% RSD on ΔH 4 |
| Multiple feedback modes | Yes (passive, high gain and low gain) |
| Temperature range | 2°C to 130°C 2, 5 |
| Maximum scan rate | 240°C/h |
| Reverse scanning | Yes |
| Pressure perturbation calorimetry (PPC) | N/A |
| Cleaning routines | 3 pre-programmed routines |
| Cleaning solvents | Water and detergent used as standard |
| 21 CFR part 11 | Yes, with PEAQ-Compliance software option |
|---|---|
| Network ready | Yes, with email alert capability |
| Operating temperature (°C) | +10°C to +28°C |
|---|---|
| Storage temperature | -20°C to +50°C |
| Humidity | 10% to 70%, non-condensing (10% to 90% for storage) |
| Ingress Protection (IP) rating | IP21 |
| Power | 100-240 V A/C, 50/60 Hz, 70 W (cell), 400 W (max, autosampler), PC as supplied |
| Certification | CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008 |
| Dimensions (W, D, H) | 101 cm × 68 cm × 70 cm |
|---|---|
| Weight | Approx. 25 kg |
| 1 Sample dependent 2 Typical results for ribonuclease (RNase) in 50 mM KAc buffer at pH 5.5, at 60°C/h with high feedback 3 Rescans of a stable buffer 4 Using ribonuclease (RNase) 5 Range may be extended down to -10°C upon request |
Protect your investment and ensure optimized performance at all times with Malvern Panalytical's service plans.
When you purchase a Malvern Panalytical product we understand that this is just the first stage of a working relationship that will last for the lifetime of the instrument. Depending on your needs, Malvern Panalytical will provide the support for your business.
Help Desk
-
We draw on the power of our analytical instruments and services to make the invisible visible and the impossible possible. Through the chemical, physical and structural analysis of materials, our high-precision analytical systems and top-notch services support our customers in creating a better world, helping them to improve everything from the energies that power us and the materials we build with, to the medicines that cure us and the foods we enjoy. We partner with many of the world’s biggest companies, universities and research organizations. They value us not only for the power of our solutions, but also for the depth of our expertise, collaboration and integrity. With over 2200 employees, we serve the world, and we are part of Spectris plc, the world-leading precision measurement group.
Malvern Panalytical. We’re BIG on small?.
| Request Information |
| Other Products |
| Related Products |
| Recently viewed products |