Features:
• Proprietary hot-start technology for unrivalled detection of low copy number templates
• Increased PCR success rates with amplicons up to 6kb
• Perfectly suited to multiplex PCR
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• Efficient specific amplification from complex templates including GC rich and AT rich sequences
• Available as a red mix for direct loading onto agarose gels
PCRBIO HS Taq DNA Polymerase uses advanced hot-start technology for superior sensitivity. Whether you need a hot-start assay for high throughput, automated reaction set up or for detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.
“Hot-start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer-dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small-molecule hot-start technology offers improved specificity and sensitivity compared to other methods.
PCRBIO HS DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HS Taq DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich). Due to enhanced efficiency and specificity the enzyme is perfectly suited to multiplex PCR.
For added convenience PCRBIO HS Taq DNA Polymerase is also available as a 2x ready mix. PCRBIO HS Taq Mix Red is suitable for direct loading on agarose gels, it contains a red dye for tracking during agarose gel electrophoresis.
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