The recombinant human Thrombopoietin protein, His Tag is expressed in Human Cells, whose amino acid sequence is derived from mature form of human THPO (NP_000451.1), (Ser 22-Gly 353) was fused with a polyhistidine tag at the N-terminus.
The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Human Thrombopoietin protein, His Tag measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 3-15 ng/ml.
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