In an exclusive agreement with 454 Life Sciences, a Roche Company, IDT is able to provide researchers with 454 Fusion Primer design software and custom primers for the Genome Sequencer FLX System.

454 Fusion Primers are required for a number of 454 Sequencing applications. These applications include "ultra-deep", amplicon sequencing for detection of low-frequency mutations, and the comprehensive identification and quantification of common and rare sequences within a population. These studies are easily initiated with 454 Fusion Primer design software to target individual exons or all exons from one or more genes.

Roche recommends IDT’s HPLC-purified 454 Fusion Primers as the best way for a researchers to ensure they are getting the best data from their sequencing runs. As with all IDT oligos, identity is guaranteed with 100% Mass Spec QC.

GS FLX Titanium Rapid Library MID Adaptor Oligos

Titanium Rapid Library MID Oligos are intended for shotgun sequencing; the MIDs are included in order to multiplex different samples on a single sequencing run.

GS FLX Titanium Rapid Library MID Adaptors Extended List

The GS FLX Titanium Rapid Library MID Adaptors Extended List includes a set of Adaptors that can be substituted for the Adaptors of the GS FLX Titanium Rapid Library Preparation Kit for the preparation of DNA libraries for sequencing on the Genome Sequencer FLX System. Tagging multiple libraries with different MIDs allows them to be amplified and sequenced together in a single region of the PicoTiterPlate device. For customers that need more MIDs than the twelve supplied by 454 in the GS FLX Titanium Rapid Library MID Adaptors Kit, this Extended List provides additional sequences that have been optimized for MID applications.

Each MID sequence contains at least 4 changes (insertion, deletion, or substitution) to make it unique from the other members of the Extended MID Set. This means that, for any of these MIDs, it is possible to either detect up to 2 errors and correct 1 error, or alternatively, detect 3 errors and correct none.

The Extended Set MIDs are sorted according to the number of reagent flows needed to sequence each, with a lower number indicating fewer flows. As a result, the lower numbered entries of the Extended Set MIDs are preferable to the higher numbered Adaptors because they can be sequenced using fewer reagent flows thereby maximizing the number of remaining flows for sequencing the library fragment.