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- Scepter™ 2.0 Cell Counter
- Product Detail
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- Precision in Cell Counting
Scepter 2.0 Cell Counter – your portable cell counter. The first and only device to bring consistency in cell counting right to the culture hood: the Scepter 2.0 Cell Counter. While other automated counters consume bench space and rely on object recognition software, manual focusing, and clumsy loading chambers, the Scepter cell counter provides true automation without the error that accompanies vision-based systems. With its microfabricated, precision-engineered sensor, the Scepter cell counter does all the work and delivers accurate and reliable cell counts in less than 30 seconds.
Scepter™ Technology for Cell Counting
Precision Cell Counting in a Portable, Personalized Format
We are constantly developing Scepter cell counting technology to expand its utility and enhance its analytical powers; the recently launched Scepter 2.0 cell counter counts more cell types and makes cell analysis easier than ever.
Scepter 2.0 does more than any cell counter on the market, making it easier than ever count your cells.
Accurately count every cell in your sample.
Qualitatively determine cell health.
Count subpopulations in Peripheral Blood Mononuclear Cells (PBMC) preps.
Easily capture, analyze and manipulate data sets with Scepter Software Pro.
Scepter™ 2.0 Cell Counter - How it Works
The gold standard counter is smaller than ever! The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Precise volumes are drawn into the Scepter™ sensor.
As cells flow through the aperture in the sensor, resistance increases, and, by Ohm’s law (V=IR, where V=voltage, I=current, and R=resistance), this increase in resistance causes a subsequent increase in voltage.
Voltage changes are recorded as spikes with each passing cell.
Spikes of the same size are bucketed into a histogram and counted.
This histogram gives you quantitative data on cell morphology that can be used to evaluate the quality and health of your cell culture.
Discover How Scepter™ Works, Step-by-Step Protocol
Sample Preparation
Start with a single-cell suspension, diluted to a total volume of 100 µL (recommended) in phosphate buffered saline (such as EmbryoMax® 1x DPBS) to 10,000-500,000 cells/mL (operating range) in a 1.5 mL microcentrifuge tube.
Counting Process
Turn on the Scepter™ cytometer by pressing the control button on the back of the instrument and wait for on-screen instructions to appear.
When prompted, attach a sensor to the end of the Scepter™ unit with the electrode sensing panel facing toward the front of the instrument, and you’ll see detailed instructions for each step of the counting process.
Pipette once to draw sample into the sensor. 50 uL of your cell suspension is drawn into the microfabricated, precision-engineered channel embedded in the sensor. The cell sensing zone detects each cell drawn into the sensor and thus cell concentration is calculated.
The sensing zone also measures cell sizes and cell volumes with sub-micron and sub-picoliter resolution, enabling the Scepter™ cytometer to display a histogram distribution of cell size or cell volume.
Gating Process
Once counting is complete, you can click the control button and scroll to manually adjust the upper and lower gates. After gating, you will see recalculated data displaying a new concentration, average cell size and volume. Gating allows you to look only at the population of cells you are interested in.
Exporting and Manipulating Data
Store up to 72 histograms on the Scepter™ cell counter itself, or upload data to your personal computer using the included software and USB cable. The Scepter™ Application Software shows uploaded volume and size distribution data as shown below. Easily share your data or conduct in-depth analysis by exporting uploaded files to Microsoft Excel.
The Scepter™ 2.0 Software Pro User Interface Has 5 Main Components
1. Data Panel– Houses all data sets used during an analysis session. Access and display previously stored data as well as files directly uploaded from a connected Scepter™ device.
2. Analysis Template Panel– Houses gating and analysis templates that may be applied to multiple data sets.
3. Current Plot– displays data from any test run selected in the Data Panel. The user can also use this plot to adjust gating position or add/remove markers. As they change, statistics are instantly updated in the Acquisition table (5).
4. Multifunction Plot– Create overlays from multiple data files. Statistics derived from this plot are displayed in the overlay table (5).
5. Group Stats Window– composed of two interchangeable displays:
Data from the current plot is displayed by the acquisition table.
Data from the multifunction plot is displayed by the overlay table.
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