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- CellStream™ Benchtop Flow Cytometer for Sensitive Cell Analysis
Capabilities Today. Flexibility for Tomorrow.
The new CellStream™ benchtop flow cytometer delivers unparalleled sensitivity and flexibility for cell and particle analysis. This compact system contains patented optics technology unique to our state-of-the-art Amnis® flow cytometers and has a fully configurable 7-laser capacity. With highly sensitive and configurable optics, researchers benefit from multiparameter detection capabilities, while maintaining the flexibility to tailor and expand the system according to their research needs and budget.
Excitation & Emission Capabilities of the 7-Laser CellStream™ Flow Cytometer
Sample Handling for Both Single Tubes & 96-Well Plates Is Standard on All Instruments
The CellStream™ flow cytometry system enables walk-away sample handling for high-throughput assays. The built-in, automated plate loader tray holds a 96-well plate and is a standard feature on all instruments. To ensure that cells stay in suspension during sampling, each well is mixed by the sample probe before sampling so there is no need for manual resuspension after loading the plate. To prevent carryover, the sample probe is washed between samples with on-board fluidics. This virtually eliminates the risk of contamination between samples and results in more efficient mixing than shaking plate holder formats or aspiration methods.
Standard on All Instruments: 96-Well Plate AutoSampler
CellStream™ Optics Technology
A Flow Cytometry System with Patented Optics and a Highly Configurable
7-Laser Capacity
The CellStream™ benchtop flow cytometer features patented Amnis® optics camera technology and the ability to configure the system with 1 to 7 lasers. This gives researchers unparalleled sensitivity plus flexibility to customize and expand the system according to research needs and budget.
Compatible with Many Common Fluorochromes and Fluorescent Reagents
Thoughtful laser and detector selection ensures that CellStream™ instruments are compatible with familiar and trusted fluorophores, as well as many of the newer dyes that offer advantages such as increased brightness or photo-stability.
CellStream™ System Architecture
Merck:/Freestyle/BI-Bioscience/Cell-Analysis/CellStream/CellStream-Optic-2.jpgOur patented time delay integration (TDI) and camera technology delivers sensitivity and expandability beyond what is possible with traditional flow cytometers.
1. Up to 7 lasers are focused in discrete locations.
2. Hydrodynamically focused cells pass through the laser-illuminated region. Fluorochromes bound to the cells are excited and emit into the collection system. Fluorescence is collected and directed toward an intermediate image plane.
3. The filter stack decomposes each of the four discrete vertical positions in the intermediate image plane into 22 separate channels of data.
4. All 22 channels fit efficiently onto a CCD (charge-coupled device) array.
CellStream™ system’s sensor contains multiple discrete collection fields using the same CCD (charge-coupled device) as patented Amnis® technology.
How CellStream™ Flow Cytometry Optics Work
Charge-Coupled Device (CCD) Camera in Time-Delay Integration
CellStream™ systems, like our state-of-the-art Amnis® flow cytometers, employ a time-delayed integration (TDI), charge-coupled device (CCD) camera. This is the same technology used in medical radiography and military applications to capture images of moving objects.
The custom camera within CellStream™ flow cytometers operates using this TDI technique, whereby a specialized detector readout mode preserves sensitivity and image quality, even with fast relative movement between the detector and the objects being imaged. The TDI detection technology of the CCD camera allows up to 1000 times more signal to be acquired from cells in flow than from conventional frame imaging approaches. Velocity detection and autofocus systems maintain proper camera synchronization and focus during the process of image acquisition.
As with any CCD, image photons are converted to photocharges in an array of pixels. During TDI operation, the photocharges are continuously shifted from pixel to pixel down the detector, parallel to the axis of flow, integrating the signal over multiple pixels. By synchronizing the photocharge shift rate with the velocity of the flowing cell, the effect is similar to physically panning a camera. With TDI, image streaking is avoided despite signal integration times that are orders of magnitude longer than those of conventional flow cytometry. Each pixel is digitized with 12 bits of intensity resolution, providing a minimum dynamic range of three decades per pixel.
CellStream™ Instrument Performance
A Flow Cytometry System with Superior Sensitivity for High Performance Cell Analysis
The CellStream™ flow cytometer offers multiparameter detection capabilities and superior sensitivity in a compact, benchtop format. With the same patent-protected optical system as our state-of-the-art Amnis® instruments, CellStream™ flow cytometers rapidly capture low-resolution cell images and transform these into high-throughput intensity data.
High Fluorescence Sensitivity: 8-peak Rainbow Beads
Fluorescence sensitivity is demonstrated below using industry standard 8-peak rainbow calibration beads. The CellStream™ flow cytometer clearly resolves all 8 peaks on every detection channel, with low MESF (Molecules of Equivalent Soluble Fluorochrome) values:
MESF <10 FITC
MESF <5 PE
How it Works
The CellStream™ system uses a proprietary CCD (charge-coupled device) camera coupled with patented Amnis® TDI (time delay integration) technology and novel laser excitation technology to deliver industry-leading performance.
A sample of fluorescently-labeled cells is aspirated into the sample probe and the cells travel down the flow stream, passing through the laser-illuminated region. Fluorochromes bound to the cells are excited and emit into the collection system. Fluorescence is collected and directed toward an intermediate image plane. The filter stack decomposes each of the 4 discrete vertical positions in the intermediate image plane into 22 separate channels of data on the CCD camera array. The low resolution images are then translated into intensity data, typical to a flow cytometer.
The CellStream™ system is flexible and ‘future-proof’ for researchers with a 1- to 7-laser capacity that offers up to 22 detection channels plus the possibility of easy, on-site upgrades should additional lasers be needed.
Small, Submicron Particle Detection
Size bead data demonstrate superior detection and discrimination of small particles on the CellStream™ platform.
Superior Small Particle Detection: Size Bead Data
Acquisition of Megamix-Plus FSC size beads containing 300, 500, and 900 nm fluorescent beads in a known ratio of 4:2:1. Instrument settings: 70 mW SSC, 10% FSC, and 200 mW 488 nm; slow speed.
Immunological Phenotyping 10-Color Assay
In this example, a 4-laser CellStream™ system accurately resolves 10 different fluorochromes within a single assay. The data below show how different immune cell populations are clearly resolved within a sample of PBMCs.
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