Abbkine CheKine™ NADP/NADPH Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NAD+/NADH), in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NADP+ /NADPH concentration in the sample.
Standard Curve of NADPH in 96-well plate assay.
The y-axis is ΔOD and the x-axis is NADPH concentration (uM).
The Abbkine CheKine™ NADP/NADPH Quantification Kit has below features:
1. Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.
2. Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.
More detailed information of Cell Metabolism Assay Kits at here.
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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.