1. Sample Preparation
The candidate of reference material was prepared by biological zymolysis methods and purify by preparative chromatography technique.
| Compound |
Molecular formula |
CAS |
| Patulin (PAT) |
C7H6O4 |
149-29-1 |
2 . Traceability and Characterization Method
Candidate material was characterized by UV spectroscopy, melting point method, and nuclear magnetic resonance. Mass balance and qNMR methods was used to assign value of the RM. In mass balance, high performance liquid chromatography with diode array detector (HPLC-PDA) was used to determine the main component. Water was determined by Karl-fisher method. Total non-volatiles/inorganics were determined by ICP-MS and TGA method. Volatile solvents were determined by qNMR and GC-ECD methods.
3. Certified values and uncertainties
The property values and expanded uncertainty of the present CRM are as follows：
| No. |
Reference material |
Certified Value (%) |
U (%) k=2 |
| GBW10257 |
Patulin (PAT) |
99.7 |
0.2 |
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