The recombinant human IL-2 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human IL-2 (P60568-1), (Ala 21-Thr 153) was expressed and purified, with an initial Met.
The recombinant human IL-2 consists of 134 amino acids and has a calculated molecular mass of 15.5 kDa as predicted in SDS-PAGE under reducing conditions.
Human IL-2 protein Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells.The ED50 for this effect is typically 1-5 ng/mL.
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