The recombinant human M-CSF protein, His Tag is expressed in Human Cells, amino acid sequence derived from N-terminal fragment (Met 1-Asn 190) of human CSF1 (P09603-1) was fused with a polyhistidine tag at the C-terminus. Human and Cynomolgus CSF1 sequences are identical.
The recombinant human CSF1 consists of 169 amino acids and predictes a molecular mass of 19.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSF1 is approximately 27-32 kDa band due to glycosylation.
Human M-CSF protein, His Tag measured by its binding ability in a functional ELISA.
1. Immobilized human CSF1R (Cat:10161-H08H) at 10 μg/mL (100 μl/well) can bind biotinylated human CSF-1 (Cat:11792-H08H). The EC50 of biotinylated human CSF-1 (Cat:11792-H08H) is 8 ng/mL.
2. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.4-2.3 ng/mL.
Abbkine suplies sizes: 5µg, 20µg, 100µg of Human M-CSF protein, His Tagfor your choice, more detailed information at https://www.abbkine.com/product/human-m-csf-protein-his-tag-prp100265.
bio-equip.cn
中文
