Chromatin can be sheared enzymatically or by mechanical methods such as sonication. The Enzymatic ChIP 96 microplate kit is a perfect alternative for those customers who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The Chromatrap^® 96 high throughput ChIP microplate for qPCR is based on the novel spin-column ChIP technique.

The kit contains 96 Protein-A or Protein-G based filters in one SLAS/ANSI standard microplate footprint device, with buffers and reagents to perform up to 96 chromatin immunoprecipitation assays and up to 10 chromatin preparations by enzymatic digestion.

For the first time this kit allows you to perform up to 96 ChIP assays through to qPCR analysis in less than one working day. Large, reliable data sets for qPCR can be collected efficiently, enabling widespread effects to be studied across multiple samples and cell types; all carried out simultaneously.

The Enzymatic 96 ChIP seq kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. Chromatrap^® Enzymatic 96 ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.

• Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
• This kit is designed for qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1-10µg).
• Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample.
• The kit is also available for sequencing as a downstream process.

Chromatrap HT Enzymatic ChIP-qPCR Protein A
Product Code : 500162

Kit Contents: Chromatrap^® 96 HT Protein A, Chromatrap^® 96 HT balance plate, 96- well collection plate, 96- well balance collection plate, 96- well elution plate, 96- well balance elution plate, Elution and balance plate strip caps, Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Digestion Buffer, Enzymatic stop solution, Lysis Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K.