Specification: 10 mL，20 mL, 50 mL, 100 mL
Concentration:（5mg/mL）
Size: 100 nm, 300 nm
Storage: Stored at 2-8°C for 6 months.
Saturation magnetization: > 10 emu/g
Application
The surface of the supper paramagnetic Fe3O4 nanoparticles is covalently functionalized with streptavidin, can be used to bind biotin-conjugated molecules, such as antibody, protein, peptide, DNA primers, etc., through high affinity interaction between streptavidin and biotin.
The Streptavidin-biotin-biomolecule complex is easy to be separated from unbound biotin-biomolecule using a magnet, therefore providing a quick and neat way to tag biomolecules with magnetic nanoparticles. The purified nanoparticle-biomolecule complex can be used in a variety of downstream bio-separation processes: protein purification, immunoprecipitation, cell isolation or depletion, and molecular detection.
Cell Enrichment
Ecalbio™ Streptavidin magnetic nanoparticles are ideal for isolation or depletion of cells (e.g. CTCs, stem cells) from a mixture of cell population obtained from tissues or organs. The isolated cells are viable and can be further cultured or used for downstream molecular analysis such as mRNA isolation and RT- PCR. Cell separation with Ecalbio™ eliminates the use of columns, so cells are not exposed to the mechanical stress from passing through the column matrix. Magnetically separated cells are highly purified and retain their viability, ideal for downstream applications.
• Easy and quick to make nanoparticle-antibody conjugates through streptavidin-biotin association
• Simple and gentle cell separation
• Consistent, high quality results
• High binding capacity
• High biocompatibility
• Low non-specific binding
Product Contents
• Ecalbio™ Streptavidin nanoparticles (Cat# 21005) are provided in phosphate buffered saline (PBS), pH 7.4. Each vial contains 10 ml of solution with a particle concentration of 5 mg /ml, which is enough for isolating approximately 500 million cells.
• Washing Buffer (10✕), Cat# A20001
• Magnet, Cat# A20003
• Ecalbio™ Streptavidin Kit, Cat# K21005
Price
| NO |
Concentration |
Specification |
Price |
| ECA1501 |
5 mg/mL |
10 mL |
1200 USD |
| ECA1502 |
5 mg/mL |
20 mL |
2200 USD |
| ECA1503 |
5 mg/mL |
50 mL |
5000 USD |
Note
Products will be developed according to customer’s requirements within 2 weeks.
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