The reactor provides a simulated intestinal environment in vitro. A fully automatic extrinsic simulation model of intestinal bacteria and an in vitro enrichment method for uncultured intestinal bacteria

The continuous fermentation process can be controlled automatically to simulate the in vitro continuous culture device of human and animal intestinal microflora, and the bacterial flora that cannot be cultured in vitro can be enriched by regulating the fermentation parameters of the model.

1. Composition:

The reactor includes a touch screen chip control system, a group of bioreactor, medium input, waste liquid output system, medium flow acceleration rate control system, liquid level control system, gas path system, temperature control system, PH regulation system, mechanical lifting system, frame, housing, water tray, etc. A fully automated intestinal model in vitro was constructed, which has the characteristics of simple operation, convenient use and good system stability.

2. Application:

1. It can be used for the study of intestinal microecology, interaction between gut and microorganisms, interaction between normal flora and pathogenic bacteria, and interaction between antibiotics and normal flora

2, can be used in the development of new drugs, probiotics and functional foods.

3. The enrichment of intestinal uncultured bacteria in vitro will be conducive to a better understanding of intestinal microbial function and lay a solid foundation for the development of new products.

4, the four sets of continuous culture system can be used alone for intestinal flora simulation culture, or the four sets of systems can be assembled into two phase, three phase or even four phase continuous fermentation system through the elevator.

3. Technical advantages:

1. The model of human and animal gut was established;

2. PCR-DGGE, 454 sequencing and short-chain fatty acid concentration determination were used to evaluate that the reactor had a good effect on the simulation of human feces and animal gut contents microflora

3. The reactor has good stability and repeatability and wide applicability.

4, can increase the proportion of uncultured bacteria in the fermentation liquid, can also selectively enrich some uncultured bacteria, which will be conducive to our better understanding of the function of intestinal microbes and the development of new products.