As a special equipment necessary in the biological reaction process, the shaking table is used for microbial, animal and plant cell strain screening, seed expansion culture, etc. Due to the characteristics of shaker equipment, it is impossible to measure the relevant parameters in the culture process and the process feeding control in real time, so the results of shaker bottling have been used as experimental data for a long time. When used as a basis for studies such as changes in the physiological characteristics of strains, the role of media components, and changes in environmental conditions such as temperature and pH, it is actually a static analysis method that lacks process studies. The limitations of this method are obvious, for example, when this method is used as a post-breeding technology for strains, the traditional shake bottle screening method often lacks feed or oxygen supply, and is not necessarily in the most reasonable state of metabolic flow distribution, which will lead to serious sieve leakage phenomenon of high-yield strains.

The series of high-throughput bioreactor produced by Shanghai Guxin Biotechnology Co., LTD., is that according to the fermentation operation of the bacteria in the metabolic flow fermenter, the process pH value and other parameters can be obviously observed (such as cell growth, OUR, CER, RQ, various feeding operations). This correlation change with different fermentation time can reflect the changes in the physiological state of the bacteria, which is of great significance for the optimization and amplification of the fermentation process. Different from the past concept of fermentation pH value as the best condition, the pH detection function of the device can observe changes in the physiological characteristics of bacteria according to the principle of correlation of fermentation process parameters. The high throughput bioreactor with pH measurement not only overcomes the difficulty of multi-parameter measurement in shaker, but also is the basic principle of metabolic flow analysis. In order to further control the culture process, the micro-feeding technology for shaking bottles is also carefully designed to realize the flow addition of various carbon and nitrogen sources, pH control or trace element control. The combination of real-time pH measurement and flow-adding technology is of great significance to the dynamic study of metabolic flow.

Due to the multi-bottle shaking bottle and the combination of different processes, the research progress can be greatly shortened. It can be seen that the high-throughput bioreactor with pH measurement and feed control is not only a simple bioreactor in essence, but also particularly suitable for the early large-scale work of screening and culture of various microorganisms and genetically engineered bacteria, and the high-throughput screening ability is obviously better than the conventional shaker and bioreactor. The difficulty of its equipment manufacturing is to develop a stable pH measuring device and a fine feeding system. Due to the high temperature sterilization resistance of autoclave and the high impedance input characteristic of shaker oscillation, the pH electrode device and wire meter system must be carefully designed. The feeding system must be small, effective, and can be programmed manually.