Factor X (FX), as known as Stuart-Prower factor or prothrombinase, is the first member of the final common pathway (thrombin pathway) in the coagulation cascade. Activated by factor IXa or factor VIIa, Factor X turn into it's activated form, factor Xa (FXa), which is capable of activating factor II (prothrombin) into thrombin.
Our FXa is prepared from bovine plasma, then activated by Russell's viper Venom-factor X activator (RVV-X), and purified by ion exchange chromatography and gel filter chromatography.
FXa is mainly used in anti-FXa activity assay of heparin.
Applications:
Factor Xa can be used in many applications, such as:
1. Anti-factor Xa activity to anti-factor IIa activity ratios of heparin and LMWHs
2. Anti-factor Xa activities of heparin and LMWHs
3. Anti-factor Xa activities of heparin and LMWHs in human plasma
4. Production of kits for automated blood analyzers
5. Evaluation of potential anticoagulants from chemical synthesis, bacterial fermentation and animal tissues
Composition:
White lyophized powder in vials.
Each vial contains: FXa 2.5 IU (50 nkat S-2222); BSA 20 mg; Tris 60.5 mg.
Physical and Chemical Properties
EC number: 3.4.21.6
Appearance: white lyophilized powder
Purity: electrophoretic purity
Molecular weight: about 44 kDa
E1cm1%(280 nm): about 12
Specific activity: not less than 90 IU/mg
Standard: 71nkat (3.55 IU)
Usage
Reconstitute the entire contents of a vial, dissolve it with 10 mL distilled water to get a solution with concentration of 0.25 IU/mL FXa, pH 7.4
Stability and Storage:
The lyophilized powder of FXa could be preserved at 2 to 8℃ for 12 months.
The 0.25 IU/mL FXa solution could be preserved at 2 to 8 ℃ for about 1 month.
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