Usage
Research reagent (glycobiology, preparation of oligosaccharide libraries from dermatan sulfate).
Determination of contents of chondrotin sulfates by HPLC.
General Information
Synonyms: Chondroitin B eliminase
Gene Source: Native Flavobacterium heparinum
EC Number: 4.2.2.19
CAS Number: 52227-83-5
MDL Number: MFCD00166444
Catalyzed Reaction: Chondroitinase B cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin B). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Substrate Specificity: Dermatan sulfate (chondroitin sulfate B).
Properties:
Molecular weight: 55kDa
Isoelectric point: 9.4 – 9.6
pH optimum for activity: 7 – 8
pH range for activity: 5 – 10
Optimal temperature range: 20 ℃ – 37 ℃
Purity: ≥ 90% by reversed phase HPLC analysis.
Standard: 1 IU and 5 IU.
Specific Activity: Greater than/equal to 200 IU/mg (substrate: dermatan sulfate). One international unit (IU) of chondroitinase B is defined as the amout of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 ℃ and pH 8.0.
Stability: 12 months froze at– 20 ℃ in aqueous buffers containing sodium phosphate and sucrose 5%.
Applications
Chondroitinase B is useful in many applications, for example:
As research reagent (glycosaminoglycan degradation).
For the preparation of di- and oligo-saccharides of dermatan sulfate.
Order Information
Catalog number: AG00-8058
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