REAGEN™Salmonella ELISA Test Kit is designed for routine screening for Salmonella from cultured food and feed samples. Salmonella is a genus of gram negative bacteria belonging to the Enterobacteriaceae family. As a major contributor to foodborne illness, Salmonella is founded in poultry,food, water,meat and eggs. When Salmonella is consumed it may result in the disease known as Salmonellosis. The detection of Salmonella in food samples is often laborious and time-consuming. Several days are required to culture the bacteria from a sample followed by a final isolation and identification by biochemical and serological techniques. The REAGEN™Salmonella ELISA Test Kit provides a simple, rapid, sensitive and cost-effective enzyme immunoassay (ELISA) screening method, which enables government agencies,seafood processors,as well as quality assurance organizations,to detect positive and negative Salmonella samples to as low as 105 cells/mL level.
Procedure Overview
The method is based on a colorimetric ELISA assay. The monoclonal antibody against Salmonella has been coated in the plate wells. During the analysis, the sample is added. If Salmonella is present in the sample, it will bind with the antibody.A suitable broth is added to the well for rapid growth.The secondary antibody, tagged with a peroxidase enzyme, targets the Salmonella that is complexed to the primary antibody coated on the plate wells. The resulting color intensity, after addition of substrate, is related to the amount of Salmonella in the sample.
Sensitivity
The kit can detect 105cells/mL
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