REAGEN™ Urea Enzymatic Assay Kit is a icolorimetric assay for the determination of urea in liquid samples. Urea Enzymatic Assay Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 20 ppm urea for milk. The linear range of the assay is 20 – 1000 ppm analyte.
The unique features of the kit are:
- Rapid and simple method
- Minimal sample prep
- High accurate and reproducible
Procedure Overview
REAGEN™ Urea Enzymatic Assay Kit measures the concentration of urea using the urease enzyme, which converts urea to ammonia.
urease
CO(NH2)2 + H2O
CO2 + 2 NH3
The ammonia produced from the urea is then directly detected by a colorimetric chemical reaction.
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