REAGEN™ Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Glucose directly from food samples.
The unique features of the kit are:
- High sensitivity and low detection limit (0.8 ppm)
- High reproducibility
- A rapid (30 minute), robust enzyme-based assay
Procedure Overview
REAGEN™ Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample.
The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.
Sensitivity (Detection Limit)
|
Sample Volume
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Detection Limit (mg/L)
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0.01 mL
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8
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0.1mL
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0.8
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The linear range of the assay is 0.8 – 1000 mg/L (ppm).
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