REAGEN™Sulfonamide ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of sulfonamide in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.
The unique features of the kit are:
- Rapid extraction method for various samples with high recovery (80-105%).
- High sensitivity (0.5 ppb) and low detection limit (0.25 ppb for shrimp).
- High reproducibility.
- A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Sensitivity
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
|
Feed
|
2.5
|
|
|
0.25
|
Fish/Shrimp/Meat/Liver/Kidney
|
1.0
|
|
Honey
|
1.0
|
|
|
2.5
|
|
|
2.0
|
|
|
2.5
|
|
|
0.25 (Rapid Extraction Method)
|
|
Urine
|
2.5
|
|
Water
|
0.25
|
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