REAGEN™DON ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of DON in wheat,barley, oats, rye, and maize.
The unique features of the kit are:
High recovery (75% - 105%), and cost-effective extraction methods .
High reproducibility.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The DON antibody has been coated in the plate wells. During the analysis, sample is added along with the DON-horseradish peroxidase (DON-HRP) conjugate. If the DON residue is present in the sample, it will compete for the DON antibody, thereby preventing the DON-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the DON residue concentration in the sample.
Sensitivity
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Sample Type
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Detection Limit (ng/g or ppb)
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Cereals/Feed(low concentration)
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125
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Cereals/Feed(high concentration)
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500
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