REAGEN™Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin in cereals, meat/fish, feed, milk ,oils,peanuts and pistachios .
The unique features of the kit are:
High recovery (80-105%),rapid(10-40minutes), cost-effective extraction methods.
High sensitivity (0.05 ng/g or ppb).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Sensitivity
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
Meat/fish/sereal/feed/seed/starch
|
0.5
|
|
Meat/liver/kidney
|
0.5
|
|
Milk
|
0.5
|
|
Milk powder
|
4.5
|
|
Peanut butte
|
0.5
|
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