REAGEN™ Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.
The unique features of the kit are:
- High recovery (70% - 95%), and cost-effective extraction methods for various samples.
- A quick ELISA assay (less than 1.5 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Nitroimidazole antibody has been coated in the plate wells. During the analysis, sample is added along with the Nitroimidazole -horseradish peroxidase (HRP) conjugate. If the target is present in the sample, it will compete for the Nitroimidazole antibody, thereby preventing the Nitroimidazole- HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of substrate((TMB), has an inverse relationship with the target concentration in the sample.
Sensitivity
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Sample Type
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Detection Limit (ng/g or ppb)
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Meat/Liver/Kidney/Fish
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0.25
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Shrimp
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0.25
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