REAGEN™Chloramphenicol (CAP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.
The unique features of the kit are:
- High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
- High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb) for shrimp, fish and meat samples.
- High reproducibility.
- A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The CAP antibody has been coated in the plate wells. During the analysis, sample is added along with the CAP-horseradish peroxidase conjugate. If the CAP residue is present in the sample, it will compete for the CAP antibody, thereby preventing the CAP-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the CAP residue concentration in the sample.
Sensitivity
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Sample Type
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Detection Limit (ng/g or ppb)
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0.25
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Fish/Shrimp/Meat
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0.025
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0.05
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0.05
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0.25
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|
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0.1
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0.04
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0.5 (rapid extraction)
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0.04
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0.05 (rapid extraction)
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