REAGEN™ Beta-Lactam ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of beta-lactams including ampicillin, penicillin G, amoxicillin, cloxacillin, oxacillin in milk, urine, tissue (liver, kidney, and muscle), serum, and plasma samples. REAGEN™ Beta-lactam ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect beta-lactam containing antibiotics to satisfy customer concerns about food safety.
The unique features of the kit are:
- High recovery (75-105%), low-cost rapid sample prep protocol (10 minutes - 1 hour)
- High sensitivity: 0.4 ng/gram = 0.4 ppb (parts per billion).
- High reproducibility (interplate and intraplate CV typically <8%)
- Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest (beta-lactam antibiotic) has been coated in the plate wells. The sample to be tested is mixed with a high-affinity beta-lactam capture protein and added to the well. If the beta-lactam target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of beta-lactam antibiotic in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the beta-lactam standard provided in the kit.
Sensitivity
|
Antibiotic
|
Milk, Meat, Tissue, Fish and Urine(ppb)
|
Serum and Plasma(ppb)
|
|
Penicillin G
|
0.40
|
0.80
|
|
Ampicillin
|
0.43
|
0.86
|
|
Azlocillin
|
0.40*
|
0.80
|
|
Pipercillin
|
0.52
|
1.04
|
|
Nafcillin
|
0.58
|
1.16
|
|
Amoxicillin
|
0.62
|
1.24
|
|
Cloxacillin
|
0.64
|
1.28
|
|
Oxacillin
|
0.64
|
1.28
|
|
Cefoperazone
|
0.67
|
1.34
|
|
Ceftiofur
|
0.7
|
1.4
|
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