REAGEN™ Amoxicillin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of amoxicillin in milk, urine, meat, tissue (liver, kidney, and muscle), serum, and plasma samples.
The unique features of the kit are:
- High recovery (>80%), low-cost rapid sample prep protocol (10 minutes - 1 hour)
- High sensitivity: 0.2 ng/gram = 0.2 ppb (parts per billion)
- Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)
- High reproducibility (interplate and intraplate CV typically <8%)
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest (amoxicillin) hasbeen coated in the plate wells. The sample to be tested is mixed with a high-affinity amoxicillin capture protein and added to the well. If the amoxicillin target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of amoxicillin in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the Amoxicillin standard provided in the kit.
Sensitivity
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Sample Type
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Detection Limit(ng/g or ppb)
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|
Meat/Fish/tissue
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2
|
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Milk
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2
|
|
Serum/Plasma
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4
|
|
Urine
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2
|
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