REAGEN™Fluoroquinolone ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect major fluoroquinolones to as low as 0.5 ppb level in various sample types and to satisfy customer concerns about food safety. Fluoroquinolone ELISA Test Kit iprovides a unique extraction method, which is accurate, rapid, cost effective and easy to use. .
The unique features of the kit are:
The extraction of fluoroquinolones from egg, honey, feed, meat, milk, tissue, serum and urine can be finished in 10 - 30 minutes with a high recovery rate of 75 - 105%.
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples..
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Sensitivity (Detection Limit)
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
|
Egg
|
1.0
|
|
Honey
|
1.0
|
|
Feed
|
1.0
|
|
Meat/Liver/Kidney/Fish/Shrimp
|
1.0
|
|
Milk
|
0.5
|
|
Serum
|
0.5
|
|
Urine
|
0.5
|
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