The recombinant human IL-9 protein, His Tag is expressed in Baculovirus-Insect Cells, whose amino acid sequence is derived from human IL9 (NP_000581.1), (Gln 19-Il4 144) was expressed and purified, fused with a polyhistidine tag at the C-terminus.
The recombinant human IL9 consists of 136 amino acids and predicts a molecular mass of 15.5 kDa. rhIL9 migrates as multiple bands with the molecular mass of 18-25 kDa band in SDS-PAGE under reducing conditions due to different glycosylation.
Human IL-9 protein, His Tag measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
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