The recombinant human FGF21 protein, His Tag is expressed in E. coli, whose amino acid sequence is derived from mature form of human FGF21 (NP_061986.1), (His 29-Ser 209) was expressed, with a polyhistide tag at the N-terminus.
The recombinant human FGF21 consisting of 188 amino acids and has a calculated molecular mass of 20.2 kDa. It migrates as an 23 kDa band in SDS-PAGE under reducing conditions as predicted.
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