The recombinant human GDNF protein is expressed in Human Cells, whose amino acid sequence is derived from mature form of human GDNF (P39905-2), (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
Human GDNF protein
1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-15 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera.
2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA.
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