Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10,000-1:100,000), IHC (1:500-1:2,000) and ELISA (1:5,000-1:100,000).
Clonality
Polyclonal
Isotype
Goat IgG
Purification
Affinity purified using solid phase Swine IgG (H&L) with finally > 95% purity based on SDS-PAGE.
Conjugate
HRP
Product Properties
Formulation
Liquid solution
Storage buffer
Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Additional Information
Background
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
Others
The antibody reacts with whole molecule swine IgG. It also reacts with heavy chains of swine IgG, and light chains of all other swine immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
bio-equip.cn
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.