Chromatrap® Enzymatic ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours.
By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
The Enzymatic ChIP kit is a perfect alternative for those who cannot get access to a sonicator to shear the DNA required for a ChIP assay. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40µls) with no DNA clean up steps prior to qPCR. This kit is designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1µg-10µg)
• Optimised for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
• The kit contains sufficient Protein-A or Protein-G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and reagents for enzymatic shearing of chromatin (up to 10 preparations)
• A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel
Premium kits containing positive and negative antibody and primer sets are available to provide you with satisfaction of chromatin preparation.
Chromatrap Premium Enzymatic ChIP qPCR Protein G
Product Code : 500169
Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Digestion Buffer, Enzymatic Stop Solution, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Shearing Cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes. The premium kit also includes Positive Antibody Control: Histone H3, Negative Antibody Control: IgG, Forward and Reverse primer set for GAPDH (human promoter region).
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