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Real Time Quantitative PCR Service(qPCR Service)
Real Time Quantitative PCR Service(qPCR Service)
Place of Origin:
China
Brand:
Shanghai ShineGene Molecular Biotech,Inc
Model:
Supplier:
Shanghai ShineGene Molecular Biotech,Inc.
Price:
Hits:
3158 
Updated:
3/21/2020
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  • Product Detail
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    Description
    Real-time quantitative PCR remains one of the most sensitive and quantitative tools for gene expression used today. Quantitative PCR(qPCR) is a powerful, highly sensitive technique that can be used to quantitate gene expression, determine gene copy number, detect SNPs, and detect DNA from viral and bacterial microorganisms. ShineGene support both SybrGreen and Taqman reaction chemistries. With Taqman chemistry, a labeled probe is included in the reaction to enhance the specificity and sensitivity of target sequence detection. With SyberGreen chemistry, amplified product is detected by its interaction with the SyberGreen fluorescent dye, which selectively binds to double-stranded DNA templates. We also offer primer and probe design services. We manage all aspects of the project from assay design to data analysis. Sample preparation (Nucleic acid isolation, whole genome amplification, etc.) is also available. You can get full service from ShineGene.

    Application
    Quantitative PCR can be applied in several key areas including:
    1.Gene Expression Analysis
    2.SNP Polymorphism Analysis
    3.Quantification of miRNA
    4.Drug Response Analysis
    5.Cell Bank Copy Number Analysis
    6.Mutation Analysis
    7.Residual DNA Analysis

    Reference
    1.Chou, Q., Russell, M., Birch, D., Raymond, J. & Bloch, W. (1992) Prevention of pre-PCR misprim­ing and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res.20:1717?C1723.
    2.Roux, K. H. (1995) Optimization and troubleshooting in PCR. PCR Methods Appl. 4:5185?C5194.
    3.Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(t)) Method.Methods 2001, 25:402-408.
    4.L D Ke, Z Chen .A reliability test of standard-based quantitative PCR: exogenous vs endogenous standards Mol Cell Probes. 2000 Apr;14(2):127-35.
    5.Weihong Liu and David A. Saint Validation of a quantitative method for real time PCR kinetics Biochemical and Biophysical Research Communications 294 (2002) 347?C353
    6.Heid, C.A., et al. Real time quantitative PCR. Genome Research 6(10), 986-94, 2002.
    7.Pusterla, N., et al. Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J. Clin. Microbiol. 37(5), 1329-31, 1999.
    8.Saito T., et al. Quantitative DNA analysis of low-level hepatitis B viremia in two patients with serologically negative chronic hepatitis B. J. Med. Virol. 58(4), 325-31, 1999.
    9.Tyagi, S., and F. R. Kramer. Molecular beacons probes that fluoresce upon hybridization. Nature Biotechnology 14(3), 303-8, 1996.
    10.Tyagi S., et al. Wavelength-shifting molecular beacons. Nature Biotechnology 18(11), 1191-96, 2000.
    11.Park S., et al. Rapid identification of Candida Dubliniensis using a species-specific molecular beacons.J. Clin. Microbiol. 38(8), 2829-36, 2000.

    Price List
    Full service (from sample to data acquisition)You can get full service from ShineGene.E-mail:master@shinegene.org.cn

    RNA Isolation from Sample

    16.00€/sample

    cDNA Synthesis

    4.00€/reaction

    qPCR

    12.00€/reaction

    Taqman Probe

    120.00€/probe

    Sybr Green I

    be free



    Time
    The turn around time from sample to data is about 30 working days.

    Sample Submission Requirements
    Please visit the following web:
    http://www.synthesisgene.com/sample.html

    If you want to get more information,please visit the following web:
    http://www.synthesisgene.com/faq.html
    bio-equip.cn
    ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

    http://www.synthesisgene.com
    http://www.shinegene.org.cn/englsih
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