Principle
Liquid-phase blocking enzyme linked immunosorbent assay (LPBE) using the neutralizing monoclonal antibody (mAb) sandwich method (M-LPBE) for detection of Foot-and-mouth disease virus (FMDV) type A antibodies was developed. The antigen-antibody reaction is carried out in a suspension (or liquid medium), and the antigen is blocked by the homologous antibodies, if present, in the test serum for subsequent detection by guinea pig serum.
The antigen molecules which are not completely blocked by the antibodies in the test serum are trapped to the wells of the ELISA plates with the pre-coated typespecific rabbit antibodies.
Antigen and background controls are added containing antigen without serum and blocking buffer respectively as described in plate layout. Subsequently, the bound antigen is traced by sero-type specific guinea pig serum and anti-guinea pig HRPO conjugate and substrate reaction is followed in a standard ELISA procedure.
Storage
At 2-8℃,12 months.
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