1. Principle
This test kit is a competitive ELISA for the quantitative analysis of SEM in the tissue, liver, honey, intestine,shrimp,fish,etc.The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with SEM concentration in the sample. This value is compared to the standard curve and the content of the corresponding SEM is subsequently obtained.
Enzyme immunoassay (ELISA) is widely used as a screening system, which is more simple, rapid, sensitive and cost-effective compared with HPLC or GC-MS.
2. Technical specifications
Sensitivity : 0.02 ppb Detect time: Approx.45 min
Detection limit:
Tissue, liver,honey, intestine,shrimp,fish ................. 0.1 ppb
Recovery rate:
Tissue, liver ...................................................... 90±10%
Honey, milk, intestine ....................................... 85±15%
Cross-reaction rate:
SEM ........................................................ 100%
AMOZ .................................................... <0.1%
AHD ..........................................................<0.1%
AOZ ....................................................... <0.1%
ECALBIO CO.,LTD
Email: info@ecalbio.com Https: //www.ecalbio.com
Tel: +86-27-87745856 Mobi: +86-18963964485
Add: HZAU, Luoshi Road, Wuhan City 430075, China.
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