★ Technical Manual

     Human Mesenchymal Stem Cell Serum-free Medium (HMSC-SFM)

★ Catalog NO: BW110020

★ Components

          490 ml of Basal Medium

              5 ml of Human Mesenchymal Stem Cell Supplement (HMSCS)

              5 ml of penicillin/streptomycin solution (P/S)

★ Product Description

      Human Mesenchymal Stem Cell Serum-free Medium (HMSC-SFM) contains defined components including essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and other supplements. The product is suitable for culturing human mesenchymal stem cells. The product is strictly aseptic without any contamination of virus or mycoplasma, and exhibits stable performance. By using this medium, human mesenchymal stem cells can be quickly expanded with minimal differentiation.

★ Instruction for Use

     It is strongly recommended that cell plates should be precoated with CELLstart™1:50 (Cat. No. A10142-01, GIBCO) in Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (Cat. No. A12858, GIBCO).

1. Dilute CELLstart™1:50 (Cat. No. A10142-01, GIBCO) in Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (Cat. No. A12858, GIBCO).

2. Add diluted CELLstart™ to culture container at a final volume per surface area of 0.078 mL/cm². Refer to table for respective culture container:             

                    Culture Container                            Surface Area (cm²)                           Volume of 1:50 Diluted CELLstart

                       6-well plate                                              9.6                                                                      750ul per well

                     12-well plate                                              3.2                                                                      250ul per well

                     24-well plate                                              2.0                                                                      160ul per well

3. Incubate in a 37°C, 5% CO₂ incubator for 2 hours. It is recommended to coat culture containers the day of use or the day before. If precoating the day before, the culture container must be stored at 2 to 8°C wrapped with Parafilm^® to avoid drying.

4. Pipette out diluted CELLstart™ from culture container and discard. Culture vessel is ready for the addition of cells.

   *Note: It is not necessary to rinse the culture container after removal of CELLstart™. The bottom of the coated culture container should have a clear and wet appearance.

5. Thaw penicillin/streptomycin solution (P/S) overnight at 2 to 8 ℃.

6. Add the above thawed solutions to the Basal Medium, gently swirl the bottle to ensure the mixture homogeneity.

7. Thaw the Human Mensenchymal Stem Cell Supplement (HMSCS) in water bath (37℃) and then add it into the above mixture to make the complete medium. 8. Store the complete medium in the dark at 4℃.

★ Product Use

    This product is for laboratory research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

★ Storage Condition

    Basal Medium should be stored at 4℃, while HMSCS and P/S solution should be stored in the dark at -20℃.