Product Usage

1) Immunoturbidimetric reagent, used as an antibody and antigen carrier

2) As a universal support material for enzyme preparation, as a carrier for Immobilized enzyme

3) Development of an ELISA kit as a carrier for secondary antibodies and HRP

4) Particle size standard
 

application area

Latex microspheres can be used as raw materials for diagnostic reagents to load antibody/antigen molecules, playing a role in signal enhancement in the application of diagnostic reagents. Through the amplification effect of the carrier, the detection sensitivity of diagnostic reagent products can be improved by several orders of magnitude. Mainly applicable in the following aspects
 

1. Immunofluorescence detection

The principle of immunofluorescence detection is to first immobilize antibodies on the surface of microspheres, and then add a second antibody labeled with fluorescence to bind to the antigen. This results in a stable fluorescence signal of the microsphere antibody antigen second antibody fluorescence labeled complex. After washing, the fluorescence signal is detected using an instrument. There is a strong correlation between the intensity of fluorescence signals and the concentration of detected antigens.

At present, immunofluorescence detection reagent products in the market are: (1) AFP assay kit (immunofluorescence method); (2) Carcinoembryonic antigen quantitative assay kit (time-resolved immunofluorescence method); (3) hepatitis B virus surface antigen assay kit (time-resolved immunofluorescence method); (4) Mycoplasma detection kit (immunofluorescence method); (5) Antinuclear antibody (ANA) Indirect immunofluorescence assay kit, etc.
 

2 latex particles enhanced immunoturbidimetry

There are two methods for enhancing immune turbidimetry using colloidal particles (PETIA). One is scattering turbidimetric detection, and the other is transmission turbidimetric detection. The basic principle of both methods is to cross link antibodies or antigens on the surface of polymer gel porous microspheres. When the cross linked microspheres with antibodies or antigens are combined with antigens or antibodies, they will quickly gather together in a short time, changing the dispersion or transmittance of the reaction solution. Moreover, there is a strong correlation between the changes in the astigmatism or transmittance (i.e. absorbance) of the reaction solution and the concentration of the tested antigen or antibody, which can reflect the concentration of the tested substance within a certain range

At present, latex immunoturbidimetric reagent products on the market using immunoturbidimetry include: (1) brain rib skin detection reagent: (2) a1 microglobulin detection reagent: (3) Glycated hemoglobin detection reagent: (4) lipoprotein a detection reagent:

(5) High sensitivity C-reactive protein detection reagent; (6) C-reactive protein test reagent: (7) anti Streptolysin "O" test reagent: (8) Retinol binding protein test reagent: (9) Rheumatoid factor test reagent:<10) 132 microglobulin test reagent, (11) neutrophil Gelatinase related lipid carrier protein test reagent:<12) D-dimer test reagent:<13) CK-MB test reagent:<14) Cystatin C test reagent: (15) Myoglobin test reagent; (16) Alpha fetoprotein test reagent: (17) Troponin test reagent: (18) heart type fatty acid binding protein test reagent; Wait a minute.
 

Enhanced ELISA response

This type of application is based on the principle of ELISA reaction, using latex microspheres with a particle size range of 60-200nm for detection using an enzyme-linked immunosorbent assay (ELISA). The difference is that traditional ELISA reactions use enzyme-linked antibodies, while this application couples enzymes and antibodies to the surface of microspheres, connecting multiple enzyme molecules and antibody molecules on the surface of a microsphere. Replacing the traditional enzyme-linked antibodies labeled with one enzyme molecule and one antibody, microspheres coupled with daytime enzyme molecules and antibody molecules enhance the ELISA reaction.
 

4 latex agglutination method

Latex agglutination method is an indirect method using latex particles as carriers; Duji test. Namely, by adsorbing or coupling soluble antigens or antibodies onto their surfaces, specific antibodies or antigens bind to them, resulting in clear agglutination of the latex particles. The agglutination reaction was carried out on a glass plate, and the results were observed with naked eyes under good light, and commonly used in thousands of Rheumatoid factor tests.
Product Catalog of Carboxyl Polystyrene Latex Microspheres

Note: Adding (C) or (N) after the product number is used to distinguish whether the surface of the microspheres is carboxyl or amino.

The following are the characteristic data of some types of microspheres using Marvin laser particle size instruments

At the same time, we compared the characteristic parameters of Marvin laser particle size instruments for three imported products from abroad