Automatic protein purification system AUTUPRE25/100/150/300

Main application fields:

Protein purification system is mainly used in the fields of monoclonal antibodies, recombinant proteins, vaccines, biochemical drugs, antibiotics, natural products and polysaccharides. For biological production

Separation and purification of the downstream process of the product.

Autopure series of protein purification system:

Product features and advantages:

– modular design, providing multiple configurations to meet the needs of special processes

-The high-precision double plunger infusion pump with a number of patented technologies ensures excellent separation reproducibility;

– the whole system is composed of biocompatible materials in contact with the liquid, which meets the requirements of FDA, uspvi and other relevant regulations;

– dad full spectrum direct reading detector avoids instability and high failure rate caused by traditional mechanical switching detector;

– the fixed wavelength detector lamp is a long-life LED lamp, which has a service life of more than 8000 hours and reduces the maintenance cost;

- patent technology UV detector flow cell, low dead volume, low peak expansion, improve the chromatographic resolution;

High sensitivity on-line pH, conductivity, UV detector, low dead volume, low peak expansion, improved chromatographic resolution;

The automatic components such as buffer selection valve, automatic injection valve and column position valve are integrated to improve the automation of purification;

It has the functions of pre column, post column, differential pressure alarm and bubble sensor detection, which greatly protects the safety of the system and chromatographic column;

The chromatographic workstation software meets the requirements of fda21cfr, Part11 / GLP / GMP

Mixing principle: dynamic mixing

Volume of mixing tank: 0.6ml, 2ml, 5ml or 15ml

Gradient accuracy: ± 0.5% (condition: 5-95%, flow rate above 2% of full scale)

Type diaphragm valve or selector valve

8 valve inlets (maximum)

Including: inlet valve, injection valve, column valve, outlet valve

Inlet valve

Inleta: 1, 2 or 10

Inletb: 1, 2 or 10

Column position valve: single column position, 5 column position or 9 column position

Outlet valve: 1 or 9

UV detector

Detector type: LED fixed wavelength or dad full spectrum read only

Number of wavelengths: 1, 2, 4 or full band

Wavelength range:

DF1: 280260 or 254nm

Dad402: 200-400nm, any two wavelengths output (software settin

Dad604: 200-600nm, any four wavelength output (software setting)

Dad604: 200-800nm, any four wavelength output (software setting)

Circulation pool:

2 mm optical path, 2 UL dead volume

5mm optical path, 6ul dead volume

10 mm optical path, 8 UL dead volume

PH & conductivity detector ptc01

Read range:

0.001-999.999 MS / cm (conductivity), 0-14 (PH)

Accuracy: conductivity: ± 0.02ms/cm or 2% (within 0.3 to 300ms / cm)

PH: ± 0.1ph (in the range of 2-12)

Maximum pressure

5MPa (conductivity) / 0.5MPa (PH)

Flow cell volume: 20ul

Temperature range: 0-100 ℃

Temperature accuracy: ± 0.5 (within 4-45 degrees)

Collector frac-02

Collection rack: 2

Maximum collection: 384 (4 96 well plates)

Collection rack type: 1.5ml, 3ml, 5ml, 8ml, 15ml or 50ml

Collection volume: 0.1-50ml

Collector delay volume:

800ul (autopure100 standard, UV flow cell – collection end),

420ul (autopure25 standard, UV flow cell collection end),

*The delay volume may vary depending on the configuration.

Bubble sensor:

Principle: ultrasonic, non-contact detection

Maximum quantity: 3, injection test, pump a test, pump B test or customized

Introduction of infusion pump

Performance characteristics

The wide flow range provides separation and purification applications from laboratory scale to pilot scale.

High precision bio inert infusion pump adopts pressure compensation technology, with low pulsation and high infusion precision. Ensure the high reproducibility of separation and purification

Unique technology

The infusion pump adopts the pressure compensation technology to reduce the pressure pulsation, deliver highly stable liquid for the protein purification system, prolong the service life of the packing, and improve the gradient accuracy

Chromatographic workstation

Cdsystem chromatographic workstation is a powerful, advanced and stable chromatographic data management system, which integrates the functions of instrument control, method management, data analysis, standard curve, report editing, user rights management, audit tracking, digital signature, network database, etc. it meets the requirements of fda21cfr, Part11 / GLP / GMP, It meets the requirements of the pharmaceutical industry for data traceability and safety.

If you have any questions, please contact: E-mail: pangjun@costones.com