中文
- Heat-labile UNG
- Product Detail
- Company Profile
- UNG activity at 20-40°C
- Inactivated at 50°C 2min
- RT-qPCR、multiplex RT-qPCR
- LAMP
Heat-labile UNG
|
Catalog # |
Pack size |
Price(USD) |
|
ZPH0502-S |
1000U(1U/ul) |
$300.00 |
|
ZPH0502-M |
10000U(1U/ul) |
$2700.00 |
|
ZPH0502-L |
50000U(1U/ul) |
$11000.00 |
Description
Heat-labile contains the equally named enzyme found in the marinebacterium.It selectively degrades uracil-containing PCR products. After performing PCR or RT-PCR using dUTP instead of dTTP, PCR products remain intact after treatment with Heat Labile Uracil–DNA Glycosylase, whereas contaminating DNA (i.e., not amplified) is degraded. Heat Labile Uracil–DNA Glycosylase is completely and irreversibly inactivated by moderate heat treatment at 50°C for 2min, allowing contamination control in RT-qPCR. The enzyme hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA but does not modify uracils in RNA.
Heat Labile Uracil–DNA Glycosylase is highly active at 20–40°C. No cofactors or divalent cations are required for activity, and the enzyme is active in most PCR and RT-PCR buffers. Although the enzyme is active a pH 6.5–9.0, the optimal pH 7.5 is in 50 mM NaCl.
Features:
Application:
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ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.
http://www.synthesisgene.com
http://www.shinegene.org.cn/englsih
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