1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Rapamycin in the tissue, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The Rapamycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Rapamycin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Rapamycin is subsequently obtained.
2. Technical specifications
Sensitivity : 5 ppb Detect time: Approx. 45 min
Detection limit:
Tissue, liver, intestine,milk .........................5.0ppb
Serum，medium solution .........................100ppb
Recovery rate:
Tissue, liver, intestine,milk ....... 85±10%
Cross-reaction rate:
Rapamycin.......................100 %
Tacrolimus......................<1%
ECALBIO CO.,LTD
Email: info@ecalbio.com
Tel: 86-27-87745856
Add : HZAU, Luoshi Road, Wuhan City 430000, China.
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