1. Principle
Tylosin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Tylosin in milk, honey, tissue ans serum.
The coupling antigens are coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Tylosin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Tylosin concentration in the sample.
2. Technical specification:
Sensitivity: 0.10ppb Detect time: Approx.45 min
Detect limit:
Chicken, duck, pork, shrimp, fish .............. 0.60ppb
Honey......................................................... 0.30 ppb
Serum and milk ......................................... 6.0 ppb
Cross-reaction rate:
Tylosin.................................................. 100%
Erythromycin .........................................1%
Recovery rate:
Tissue .......................................... 85 ± 10%
Honey, milk ................................ 85 ± 15%
ECALBIO CO.,LTD
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Add: HZAU, Luoshi Road, Wuhan City 430075, China.
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