Thrombin, also named factor IIa (FIIa), is a serine protease present in the coagulation cascade. Thrombin activates factor XI, VIII, V, XIII and fibrinogen.
Our α-thrombin is a resultant of bovine plasma prothrombin, activated by FXa with the aid of FVa and phospholipid, and purified by ion exchange chromotography and gel filter chromotography.
It is mainly used in anti-FIIa activity assay of heparin.
Applications:
Alpha-thrombin can be used in many applications, such as:
1. Anti-factor Xa activity to anti-factor IIa activity ratios of heparin and LMWHs
2. Anti-factor IIa activities of heparin and LMWHs
3. Anti-factor IIa activities of heparin and LMWHs in human plasma
4. Production of kits for automated blood analyzers
5. Evaluation of potential anticoagulants from chemical synthesis, bacterial fermentation and animal tissues
6. Separation and purification of other coagulation factors, growth factors and recombinant fusion proteins
Composition:
White lyophized powder in vials:
Each vial contains: α-thrombin 50 IU; BSA 20 mg; Tris 60.5 mg.
Physical and Chemical Properties:
EC Nuumber: 3.4.21.5
Appearance: white lyophilized powder
Purity: > 95%, by SDS-PAGE
Molecular weight: about 36 kDa
E1cm1%(280 nm): about 10
Isoelectronic point: 7
Specific activity: not less than 700 IU/mg protein
Standard: 50 IU
Usage:
Reconstitute the entire contents of a vial, dissolve it with distilled water or buffer to get an appropriate solution.
Stability and Storage:
The lyophilized powder of alpha-thrombin could be preserved at 2 to 8 C for 12 months.
The 5 IU/mL α-thrombin solution could be preserved at 2 to 8 ℃ for about 7 days.
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