Primers and probes for the detection of the new 2019 coronavirus(Sars-CoV-2),ShineGene_specification/price/image

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Primers and probes for the detection of the new 2019 coronavirus(Sars-CoV-2) are available from ShineGene!

The new coronavirus (2019-nCoV) was first detected in Wuhan City, Hubei Province China. Tens of thousands of infections with 2019-nCoV have been reported in China. The virus is spreading from person-to-person in parts of the world. Coronaviruses are a large family of viruses that are common in many animals, including camels, cattle, cats, and bats. However, some animal coronaviruses can infect people and spread between people, for example MERS, SARS, and now with 2019-nCoV {CDC}.

Corona virus outbreaks in January 2020.
Primers and probes recommended by the CDC & WHO
ShineGene can provide the primer and probe sequences recommended by the CDC and the WHO to researchers working on the coronavirus.
The sequences were publically provided by the CDC here and the WHO here. Researchers can order these primers and probes to create a panel in their lab, following the panel instructions from the CDC.
These materials are provided for research purposes only.
ShineGene also offer the corresponding positive plasmid.

Name	Country	Label 5’	Oligonucleotide Sequence (5’>3’)	Label 3’	Position	Working Conc. [μM]
			N Protein
2019-nCoV_N1-F	USA		GACCCCAAAATCAGCGAAAT		28287-28306	20
2019-nCoV_N1-R			TCTGGTTACTGCCAGTTGAATCTG		28335-28358	20
2019-nCoV_N1-P		FAM	ACCCCGCATTACGTTTGGTGGACC	BHQ1	28309--28332	5
2019-nCoV_N2-F			TTACAAACATTGGCCGCAAA		29164-29183	20
2019-nCoV_N2-R			GCGCGACATTCCGAAGAA		29213-29230	20
2019-nCoV_N2-P		FAM	ACAATTTGCCCCCAGCGCTTCAG	BHQ1	29188--29210	5
2019-nCoV_N3-F			GGGAGCCTTGAATACACCAAAA		28681-28702	20
2019-nCoV_N3-R			TGTAGCACGATTGCAGCATTG		28732-28752	20
2019-nCoV_N3-P		FAM	AYCACATTGGCACCCGCAATCCTG	BHQ1	28706-28727	5
2019-nCoV-NF	China		GGGGAACTTCTCCTGCTAGAAT		28881-28902	0.6
2019-nCoV-NR			CAGACATTTTGCTCTCAAGCTG		28958-28979	0.8
2019-nCoV-NP		FAM	TTGCTGCTGCTTGACAGATT	TAMRA	28934-28953	0.1
HKU-NF	Hong Kong		TAATCAGACAAGGAACTGATTA		29145-29166	0.6
HKU-NR			CGAAGGTGTGACTTCCATG		29236-29254	0.8
HKU-NP		FAM	GCAAATTGTGCAATTTGCGG	TAMRA	29196-29177	0.1
NIID_2019-nCOV_N_F2	Japanese		AAATTTTGGGGACCAGGAAC		29142-29161	0.5
NIID_2019-nCOV_N_R2			TGGCAGCTGTGTAGGTCAAC		29280-29299	0.7
NIID_2019-nCOV_N_P2		FAM	ATGTCGCGCATTGGCATGGA	BHQ1	29239-29258	0.2
WH-NIC N-F	Thailand		CGTTTGGTGGACCCTCAGAT		28320-28339	0.4
WH-NIC N-R			CCCCACTGCGTTCTCCATT		28358-28376	0.4
WH-NIC N-P		FAM	CAACTGGCAGTAACCA	MGB	28341-38356	0.1
			E gene
E_Sarbeco_F1	Germany		ACAGGTACGTTAATAGTTAATAGCGT		26269-26294	0.4
E_Sarbeco_R2			ATATTGCAGCAGTACGCACACA		26360-26381	0.4
E_Sarbeco_P1		FAM	ACACTAGCCATCCTTACTGCGCTTCG	BHQ1	26332-26357	0.2
CoV-EFP	China		ACTTCTTTTTCTTGCTTTCGTGGT		26295-26318	20
CoV-ERP			GCAGCAGTACGCACACAATC		26357-26376	20
CoV-EP		FAM	CTAGTTACACTAGCCATCCTTACTGC	BHQ1	26326-26351	5
			ORF1ab
2019-nCoV-OFP	China		CCCTGTGGGTTTTACACTTAA		13342-13362	0.6
2019-nCoV-ORP			ACGATTGTGCATCAGCTGA		13442-13460	0.8
2019-nCoV-OP		FAM	CCGTCTGCGGTATGTGGAAAGGTTATGG	BHQ1	13377--13404	0.1
HKU-ORF1b-nsp14F	Hong Kong		TGGGGYTTTACRGGTAACCT		18778-18797	0.6
HKU- ORF1b-nsp14R			AACRCGCTTAACAAAGCACTC		18889-18909	0.8
HKU-ORF1b-nsp141P		FAM	TAGTTGTGATGCWATCATGACTAG	TAMRA	18849--18872	0.1
RdRP_SARSr-F2	Germany		GTGARATGGTCATGTGTGGCGG		15431-15452	0.6
RdRP_SARSr-R1			CARATGTTAAASACACTATTAGCATA		15435-15460	0.8
RdRP_SARSr-P2		FAM	CAGGTGGAACCTCATCAGGAGATGC	BHQ1	15470-15494	0.1
Name		Label 5’	Oligonucleotide Sequence (5’>3’)	Label 3’		Working Conc. [μM]
Internal standard			RNAse Protein/NM_006413.5
RP-F	Recognized		AGATTTGGACCTGCGAGCG		28-46	20
RP-R			GAGCGGCTGTCTCCACAAGT		73-92	20
RP-P RNAse P		FAM	TTCTGACCTGAAGGCTCTGCGCG	BHQ1	49-71	5

Note:
In order to prevent any cross-contamination of primers and probes with control plasmids, our standard procedure is to spatially separated our production of oligos and genes/plasmisds by separate rooms and air systems!
Publications
1.(2020) 2019 Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
2.(2020) 2019-Novel coronavirus (2019-nCoV) real-time rRT-PCR panel primers and probes. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
3.(2020) Real-time RT-PCR panel for detection 2019-novel coronavirus. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
4.(2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].

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