Recombinant Trypsin (Liquid)--Pharmacopoeia Grade,YaxinBio_specification/price/image

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Recombinant Trypsin (Liquid)--Pharmacopoeia Grade

Cat.No.: RPT0204

CAS: 9002-07-7

EC: 3.4.21.4

Source: Recombinant Trypsin (Porcine), produced by genetic engineering, have the same amino acid sequence as the porcine pancreas trypsin.

1.Product Introduction

Trypsin specifically cleaves C-terminal peptide bonds of lysine and arginine, which can degrade intercellular binding proteins. The amino acid sequence of the Recombinant Trypsin produced by Yaxin Bio is the same as that of porcine pancreas-derived trypsin, and is produced by recombinant E. coli expression. It can replace traditional extraction of trypsin for cell digestion in vaccines, stem cells, immune cell therapy, drug screening, antibodies and other fields. Aprotinin, soybean trypsin inhibitor, etc. can inhibit enzyme activity.

2.Product Advantage

In accordance with Pharmacopoeia standards	The product is in accordance with the relevant standards of recombinant trypsin in Pharmacopoeia.
Animal origin free	The product is produced by recombination and no any animal source material is used in the production process. No exogenous virus contamination, safe to use.
High purity	The product is produced by recombination, no miscellaneous enzyme. Host protein and DNA residues are in accordance with pharmacopoeia standards.
High cell digestibility	High purity. The effect of impurities on cell growth is avoided. High specific activity lead to lower amount of trypsin needed to add for achieving the desired digestion effect.
Stable quality	Stable and continuous batch supply, little difference between batches of products, stable quality. Production process follows NSF ISO 9001:2015 quality system and complies with GMP guidelines and provides relevant regulatory support documents.

3.Product Features

Items	Specification
1.Appearance	Clear, colorless or shallow yellow liquid
2.Identification	Positive
3.Protein Concentration	70±10 mg/ml。
4.(RP-HPLC)	β-trypsin≥70%，α-trypsin ≤20%
5.(Specific Activity)	≥3800 (U/mg protein)
6.E. Coli host Cell protein residue	≤0.01%
7.Host Cell DNA residues	≤ 10 ng/mg

Activity unit: 25℃, pH7.6, 3.2 ml reaction solution(1 cm light path), one trypsin unit (USP) was defined as an increase of 0.003 in the absorption value at 253 nm by enzymatic hydrolysis of BAEE per minute.

4.Product instruction

1. Solution preparation: Melt Recombinant Trypsin at room temperature, take an appropriate amount of trypsin according to the protein content of COA, and add HBSS equilibration solution (or other buffer suitable for cell digestion. If necessary, add EDTA to a final concentration of 0-1mM, It is best not to exceed 2 mM). The recommended trypsin concentration is about 0.1-0.3mg / ml (concentration adjusted according to different cells), and gently mixed; this step can be operated at room temperature

2. Use a 0.22 μm filter to filter the trypsin solution and transfer it to a sterile container; this step can be operated at room temperature;

3. The filtered trypsin solution should be used directly as required on the day (for example, adding 1ml to T25 bottles, digesting cells at 37 °C), and the liquid can be stored at 2-8 °C for 1-2 weeks.

4. Long-term storage: If the prepared recombinant phosphatase cell digestion solution needs to be stored for a long time, it can be stored stably at -20 °C for 12 months.

5.Stability

Stability of storage: The recombinant trypsin solution was stored under -20℃ and was stable for 12 months;

Stability of transportation: Dry ice insulation transportation, won’t melt during transportation, stable activity.

6.Product Usage

For cell culture:

1. Tissue block digestion, primary cell acquisition;

2. Passage digestion of adherent cells;

3. Cell culture by microcarrier method;

4. Gently digesting stem cells;

5. Immune cell therapy, etc.

For recombinant protein:

1. Recombinant insulin production;

2. Protein sequencing, peptide mapping;

3. Specific proteolytic processes such as proteomics research.