REAGEN™Furazolidone (AOZ) ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of Furazolidone in fish, shrimp, meat (chicken,beef, pork and hepar), eggs, honey.
The unique features of the kit are:
- High recovery (75 - 105%), and cost-effective extraction methods for various samples.
- High sensitivity (0.02ng/g or ppb) and low detection limit (0.04 ng/g or ppb) for various samples.
- A quick ELISA assay (less than 1 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and the AOZ antibody are added along with the secondary antibody, tagged with a peroxidase enzyme. If the AOZ residue is present in the sample, it will compete for the AOZ antibody, thereby preventing the antibody from binding to the drug attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AOZ residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Furazolidone (AOZ) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
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Kit Contents
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Amount
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Storage
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AOZ-coated Microtiter Plate
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96-well plate (8 wells x 12 strips)
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2-8°C
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