REAGEN™ Nitrofurazone (SEM)) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Nitrofurazone in fish, shrimp ,eggs, honey and feed.
The unique features of the kit are:
- high recovery (75%-115%), and cost-effective extraction methods for various samples.
- High sensitivity (0.025 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for various samples.
- High reproducibility.
- A quick ELISA assay (less than 1 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The SEM-BSA has been coated in the plate wells. During the analysis, sample and the SEM antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the SEM residue is present in the sample, it will compete for the SEM antibody, thereby preventing the antibody from binding to the SEM-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the SEM residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Nitrofurazone (SEM) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
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Kit Contents
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Amount
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Storage
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SEM-BSA-Coated Plate
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96-well plate (8 wells x 12 strips)
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2-8°C
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SEM Standards:
Negative control (white cap tube)
0.025 ng/mL (yellow cap tube)
0.1ng/mL (orange cap tube)
0.4 ng/mL (pink cap tube)
0.8 ng/mL (purple cap tube)
1.6 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
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1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
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2-8°C
2-8°C
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SEM Antibody
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6 mL
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2-8°C
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HRP Conjugate
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6 mL
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10X Sample Extraction Buffer **
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25 mL
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20X Wash Solution **
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30 mL
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