Advantages of Immune Library:
- Higher chance to get desired VHH or VNAR single domain antibody
- Better affinity and specificity
- Easier to acquire antibodies recognizing different antigen epitomes
Disadvantages:
AlpaLife nanobody discovery services include analysis of customer-provided antigen, antigen modification, animal immunization, library construction and screening, delivery of bacterial glycerol stock and antibodies sequences.
Workflow:
Phase I. Animal immunization. The phase includes antigen modification for immunization, immunization of one animal (alpaca camel or shark) with client-provided antigen, blood collection (20 ml), and ELISA analysis, PBMC isolation and preservation. Turnaround period: 8-12 weeks.
Phase II. Phage Display Library Construction: Antibody cDNA generation via RNA isolation, PCR amplification, and diverse antibody library construction. Turnaround period: 3 weeks.
Phase III. Library Screening: Immune library Phage display screening, ELISA verification, identification and evaluation of 2-6 positive clones, antibody sequences. Turnaround period: 6 weeks.
Requirements for provided antigens： polypeptide antigen: LC/MS analysis report, purity > 90 %, amount 10 mg: protein antigen: SDS-PAGE report, purity > 85 %, amount 10 mg, concentration > 0.5 mg/ml
AlpaLife offers all services at fixed prices! No more float prices, no more hidden fees or charges. only unified fixed prices, regardless of your project details or purposes, for both research or commercial uses.
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