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Eshmuno® P Chromatography Resins
Eshmuno® P Chromatography Resins
Place of Origin:
Germany
Brand:
Merck
Model:
Merck
Supplier:
Merck KGaA
Price:
Hits:
1765 
Updated:
10/12/2018
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  • Product Detail
  • Company Profile
    High performance, acid and alkaline resistant affinity chromatography resins designed for the removal of anti-A and anti-B antibodies from plasma-derived immunoglobulin (Ig).

    Trace amounts of anti-A and anti-B isoagglutinins in plasma- derived immunoglobulin (Ig) have been associated with increased patient risk for hemolysis, a serious and sometimes fatal complication.

    Eshmuno® P anti-A and Eshmuno® P anti-B are two distinct affinity based chromatography resins specifically designed to effectively remove anti-A and anti-B isoagglutinins, respectively.

    Key Advantages:

    Reduced patient risks
    Improved economics
    Operational flexibility
    Improved quality control

    Proven Technology
    Eshmuno® P resins leverage the proven technology of the highly stable Eshmuno® base matrix coupled with target specifi ligands. Eshmuno® P resins are synthesized via immobilization of trisaccharide blood group antigens (A & B) on to the Eshmuno® base matrix, which is a rigid and hydrophilic polymer based on polyvinylether.

    Reduced Patient Risk
    Eshmuno® P resins help reduce the risk of hemolytic reactions by reducing the levels of anti-A and anti-B isoagglutinins in the final product.

    Both resins show excellent anti-A and anti-B removal capacity: Greater than 75% of anti-A and anti-B removed between pH 5 through pH 9. Refer to fi e 1 below.

    Improved Economics
    The ability to reuse chromatography resins is important for designing cost effective purification processes. Such reuse is enabled by stability of the media in routine cleaning solutions. Eshmuno® P resins can be used for multiple cycles employing acid or alkaline cleaning without loss of performance.

    Studies for reuse after cleaning-in-place (CIP) were simulated by exposing the Eshmuno® P resins to two common CIP solutions for 200 hours. The solutions were 0.5 M NaOH and a solution of 120 mM phosphoric acid, 167 mM acetic acid and 2.2% benzyl alcohol, which is typically referred to as PAB. The stability of the resins and therefore, the ability to reuse was confirmed by testing the anti-A and anti-B removal capacity of the resins before and after the exposure to the CIP solutions.

    Both Eshmuno® P resins show minimal to no reduction in the removal capacity up to 200 hours of exposure to 0.5 M NaOH and PAB solutions enabling reuse for multiple cycles depending upon the cycle definition.

    Operational Flexibility
    Eshmuno® P resins show excellent removal of anti-A and anti-B issoagglutinins with high product yields irrespective of flow rates. This translates into high productivity with operational fl y for Eshmuno® P resins.

    The intrinsic rigidity of Eshmuno® P base matrix ensures a linear relationship between back pressure and fl rates throughout the standard range of operating conditions. The recommended range of compression factor is 1.11 to 1.17.

    Improved Quality Control
    The classical agglutination assays used to measure anti-A and anti-B levels are known to be highly variable due to the nature of the test and the test samples. To avoid the inconsistency of conventional assays, we developed an innovative assay to test for the resin performance.

    This novel assay is used for the routine quality control testing of Eshmuno® P resins. The assay is highly simplified, and involves measuring the depletion of the IgM after incubation with respective Eshmuno® P resins using UV spectroscopy. The applicability of this method is confirmed by establishing a correlation between IgM binding capacity and ability to remove anti-A or anti-B Ig.

    A linear relationship is observed between the resin’s anti-A and anti-B IgM binding capacity and the ability to remove anti-A and anti-B IgG, respectively. Resins with IgM binding capacity of ≥ 12 mg/ml show higher than 75% removal in case of both anti-A and anti-B IgG.
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