BiFC System:
- Split fluorescent protein (YFP)
The system is suitable for the study of protein-protein interaction with strong protein-protein interaction. The fluorescence intensity of YFP is controlled at a low level and is not interfered by background and other factors.
- Split fluorescent protein (YFP) enhanced
The system is suitable for the study of protein-protein interaction with weak interaction, such as membrane protein interaction. YFPn and YFPc are edited manually, and YFP fluorescence is enhanced, which is convenient for observing the weak interaction signal.
- The Split luciferase (LUC) system
The system can be used to observe and take pictures of living system, especially for quantitative analysis and comparison of interaction strength. It is suitable for the study of protein interaction influenced by third-party substances. For example, the strength of protein interaction can be regulated by hormones and small molecular compounds, and the change of interaction strength can be determined by the observation of exogenous hormone compounds.
https://www.profacgen.com/bimolecular-fluorescent-complimentary-bifc.htm
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