E3 ubiquitin ligase is an enzyme that plays a key role in ubiquitin proteasome pathway. Aberrant E3 ligases have been implicated in several diseases and are widely recognized as attractive targets for drug discovery. Therefore, the analysis of E3 ligase activity have a great significance in both biochemical studies and drug discovery efforts.
The detection of E3 ligase activity often utilizes its auto-ubiquitination ability, which is a characteristic exists on almost all E3 ligase. Here we provide several methods to determine the E3 ligase activity in vitro:
https://www.profacgen.com/e3-ligase-activity-assay.htm
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